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KMID : 0545120110210070753
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 7 p.753 ~ p.756
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Characterization of Methane Oxidation by a Methanotroph Isolated from a Landfill Cover Soil, South Korea
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Lee Eun-Hee
Yi Tae-Woo
Moon Kyung-Eun
Park Hyun-jung
Ryu Hee-Wook
Cho Kyung-Suk
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Abstract
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A methane-oxidizing bacterium was isolated from the enriched culture of a landfill cover soil. The closest relative of the isolate, designated M6, is Methylocystis sp. Based on a kinetic analysis, the maximum specific methane oxidation rate and saturation constant were 4.93 mmol·gdry cell weight-1·h-1 and 23 μM, respectively. This was the first time a kinetic analysis was performed using pure methanotrophic culture. The methane oxidation by M6 was investigated in the presence of aromatic (m- and pxylene and ethylbenzene) or sulfur (hydrogen sulfide, dimethyl sulfide, methanthiol) compounds. The methane oxidation was inhibited by the presence of aromatic or sulfur compounds.
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KEYWORD
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Methylocystis sp., Methane oxidation, Aromatic compound, Sulfur compounds
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